Native Page

Productinformatie

Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Native polyacrylamide gel electrophoresis is an electrophoretic separation method typically used in proteomics and metallomics. Native PAGE separations are run in non-denaturing conditions. Detergents are used only to the extent that they are necessary to lyse lipid membranes in the cell. Complexes remain--for the most part--associated and folded as they would be in the cell. One downside, however, is that complexes may not separate cleanly or predictably, since they cannot move through the polyacrylamide gel as quickly as individual, denatured proteins.